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PCR RUO

2X Taq PCR Master Mix

A ready-to-use 2X mix of Taq DNA polymerase, dNTPs, MgCl2, reaction buffer and loading dye. Add template, primers and water, cycle, and load straight onto the gel.

Specifications

Format2X master mix with blue and yellow tracking dyes
ContainsTaq DNA polymerase, dNTPs, MgCl2, optimised reaction buffer, loading dye, density agent
Use per reaction12.5 µL mix in a 25 µL reaction
Amplicon lengthRoutine up to about 3 kb
Extension rateAbout 1 kb per minute at 72 °C
Pack sizes1 mL (about 40 reactions), 5 × 1 mL (about 200), 25 mL bulk (about 1,000)
Storage−20 °C; stable through normal freeze-thaw use
Shelf life12 months from date of manufacture, see the CoA for your lot
LabellingFor Research Use Only

Values shown are typical. The batch Certificate of Analysis shipped with your order carries the exact figures for your lot.

Where labs use it

  • Colony PCR and routine genotyping
  • Checking cloning inserts and plasmid constructs
  • Teaching labs and high-throughput screening where loading-dye mixes save a step

What the Certificate of Analysis covers

  • Lot number and date of manufacture
  • Unit definition and activity test result
  • Absence of nuclease and contaminating DNA
  • Functional PCR test with a control template and band size
  • Storage and expiry
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